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#pathogen

2 posts1 participant0 posts today
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Mx Verda

X is terrible, but after the Meta news on explicitly allowing transphobia / transmisia, overall it's just been a tough week.
Idk what I have but my asthma attack started last week. Elevated neutrophils indicate bacterial infection, my GP said? So either it was "probably viral" like the OoH 111 GP said and became bacterial as well, or I was unlucky that it was bacterial to begin with.

I've been on Prednisolone, a corticosteroid since the 9th; then last night I started Doxycyline, an antibiotic, and Montelukast.
Montelukast is a leukotriene receptor antagonist, so it blocks leukotrienes, my patient pamphlet says.

Got a chest X ray out of it, at least. (Had back pain behind my ex-gallbladder since its removal anyway.)
Let this be a lesson not to lessen your mask discipline or stubbornness when other people peer pressure you to take risks with your health.
This is my first asthma exacerbation in a decade and I do not want to continue experiencing this. I do not recommend it. The people telling me I was 'paranoid', 'over-thinking it', 'worried about nothing' and more do NOT have to feel what I am feeling.

Teenage boys in public who choose to whine at me and intentionally coughing towards me (what?!!?) is super aggressive, creepy, entitled, and harmful to everyone witnessing that. Still, my upper and mid back has not stopped aching. I don't get fevers and I don't have a cough. Only you know your body.

Some people choose to care about harm reduction; others don't. Do right by yourself and do your sustainable best by others. I believe in you!

youtube.com/watch?v=My_wEXmNgt

YouTubeThe Viruses Are Hitting Hospitals And EMS Really Hard This YearBy Data Report
#sick#illness#RespiratoryIllness
Public *
NSW.Biodiversity

Mould shuts popular Glass House Mountains National Park to climbing

"The mould spreads by any activity that moved soil, water, or plant material...These include bushwalking, mountain biking, four-wheel-driving, road construction, timber harvesting, mining, and feral pig incursions...To be honest, people are going to the toilet up on top of the mountains."
>>
abc.net.au/news/rural/2024-10-
#Phytophthora #soil #pathogen #sprawl #Tourism #climbing #compulsion #roads #logging #cars #faeces #respect #EndTheClimb #biosecurity #mountain #harm

ABC News · Phytophthora microscopic mould shuts popular Glass House Mountains trail as a national biosecurity risk By Amy Sheehan
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Galaxy-Team der Uni Freiburg

🌍 Impact
PathoGFAIR is set to improve pathogen tracking, providing robust tools for researchers and public health officials worldwide. By improving detection capabilities, we can better protect public health. 🌎🔬

📝 Preprint: biorxiv.org/content/10.1101/20
🔗 Blogpost: galaxyproject.org/news/2024-07

@bebatut
#metagenomics #pathogen #UseGalaxy (6/6)

bioRxiv · PathoGFAIR: a collection of FAIR and adaptable (meta)genomics workflows for (foodborne) pathogens detection and trackingBackground Food contamination by pathogens poses a global health threat, affecting an estimated 600 million people annually. During a foodborne outbreak investigation, microbiological analysis of food vehicles detects responsible pathogens and traces contamination sources. Metagenomic approaches offer a comprehensive view of the genomic composition of microbial communities, facilitating the detection of potential pathogens in samples. Combined with sequencing techniques like Oxford Nanopore sequencing, such metagenomic approaches become faster and easier to apply. A key limitation of these approaches is the lack of accessible, easy-to-use, and openly available pipelines for pathogen identification and tracking from (meta)genomic data. Findings PathoGFAIR is a collection of Galaxy-based FAIR workflows employing state-of-the-art tools to detect and track pathogens from metagenomic Nanopore sequencing. Although initially developed for foodborne pathogen data, the workflows can be applied to any metagenomic Nanopore pathogenic data. PathoGFAIR incorporates visualisations and reports for comprehensive results. We tested PathoGFAIR on 130 benchmark samples containing different pathogens from multiple hosts under various experimental conditions. Workflows have successfully detected and tracked expected pathogens at least at the species rank in both pathogen-isolated and non-pathogen-isolated samples with sufficient Colony-forming unit and Cycle Threshold values. Conclusions PathoGFAIR detects the pathogens or the subspecies of the pathogens in any sample, regardless of whether the sample is isolated or incubated before sequencing. Importantly, PathoGFAIR is easy to use and can be straightforwardly adapted and extended for other types of analysis and sequencing techniques, making it usable in various pathogen detection scenarios. PathoGFAIR homepage: <https://usegalaxy-eu.github.io/PathoGFAIR/> ### Competing Interest Statement The authors have declared no competing interest. * ### List of Abbreviations AMR : Antimicrobial resistance API : Application Programming Interface CFU : Colony-forming unit Ct : Cycle Threshold EFSA : European Food Safety Authority EU : European Union FAIR : Findable Accessible Interoperable Resulable GTN : Galaxy Training Network IWC : Intergalactic Workflow Commission MLST : Multilocus sequence typing NGS : Next-Generation Sequencing QC : Quality Control RKI : Robert Koch Institute SNP : Single-nucleotide polymorphism SRA : Sequence Read Archive VF : Virulence Factor VFDB : Virulence Factor database WHO : World Health Organization WGS : Whole Genome Sequencing
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