#metabarcoding

Teresita Porter 🙋🏻‍♀️Applying environmental DNA metabarcoding to calculate an index of biotic integrity for freshwater fish<a href="https://www.biorxiv.org/content/10.1101/2025.05.26.656190v1" rel="nofollow noopener" translate="no" target="_blank">https://www.biorxiv.org/content/10.1101/2025.05.26.656190v1</a><a href="https://ecoevo.social/tags/environmentalDNA" class="mention hashtag" rel="nofollow noopener" target="_blank">#environmentalDNA</a> <a href="https://ecoevo.social/tags/eDNA" class="mention hashtag" rel="nofollow noopener" target="_blank">#eDNA</a> <a href="https://ecoevo.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> <a href="https://ecoevo.social/tags/freshwater" class="mention hashtag" rel="nofollow noopener" target="_blank">#freshwater</a> <a href="https://ecoevo.social/tags/fish" class="mention hashtag" rel="nofollow noopener" target="_blank">#fish</a> <a href="https://ecoevo.social/tags/IndexOfBioticIntegrity" class="mention hashtag" rel="nofollow noopener" target="_blank">#IndexOfBioticIntegrity</a>

nf-corePipeline release! nf-core/ampliseq v2.14.0 - Ampliseq Version 2.14.0!Please see the changelog: <a href="https://github.com/nf-core/ampliseq/releases/tag/2.14.0" rel="nofollow noopener" translate="no" target="_blank">https://github.com/nf-core/ampliseq/releases/tag/2.14.0</a><a href="https://mstdn.science/tags/16s" class="mention hashtag" rel="nofollow noopener" target="_blank">#16s</a> <a href="https://mstdn.science/tags/18s" class="mention hashtag" rel="nofollow noopener" target="_blank">#18s</a> <a href="https://mstdn.science/tags/ampliconsequencing" class="mention hashtag" rel="nofollow noopener" target="_blank">#ampliconsequencing</a> <a href="https://mstdn.science/tags/edna" class="mention hashtag" rel="nofollow noopener" target="_blank">#edna</a> <a href="https://mstdn.science/tags/illumina" class="mention hashtag" rel="nofollow noopener" target="_blank">#illumina</a> <a href="https://mstdn.science/tags/iontorrent" class="mention hashtag" rel="nofollow noopener" target="_blank">#iontorrent</a> <a href="https://mstdn.science/tags/its" class="mention hashtag" rel="nofollow noopener" target="_blank">#its</a> <a href="https://mstdn.science/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> <a href="https://mstdn.science/tags/metagenomics" class="mention hashtag" rel="nofollow noopener" target="_blank">#metagenomics</a> <a href="https://mstdn.science/tags/microbiome" class="mention hashtag" rel="nofollow noopener" target="_blank">#microbiome</a> <a href="https://mstdn.science/tags/pacbio" class="mention hashtag" rel="nofollow noopener" target="_blank">#pacbio</a> <a href="https://mstdn.science/tags/qiime2" class="mention hashtag" rel="nofollow noopener" target="_blank">#qiime2</a> <a href="https://mstdn.science/tags/rrna" class="mention hashtag" rel="nofollow noopener" target="_blank">#rrna</a> <a href="https://mstdn.science/tags/taxonomicclassification" class="mention hashtag" rel="nofollow noopener" target="_blank">#taxonomicclassification</a> <a href="https://mstdn.science/tags/taxonomicprofiling" class="mention hashtag" rel="nofollow noopener" target="_blank">#taxonomicprofiling</a> <a href="https://mstdn.science/tags/nfcore" class="mention hashtag" rel="nofollow noopener" target="_blank">#nfcore</a> <a href="https://mstdn.science/tags/openscience" class="mention hashtag" rel="nofollow noopener" target="_blank">#openscience</a> <a href="https://mstdn.science/tags/nextflow" class="mention hashtag" rel="nofollow noopener" target="_blank">#nextflow</a> <a href="https://mstdn.science/tags/bioinformatics" class="mention hashtag" rel="nofollow noopener" target="_blank">#bioinformatics</a>

Teresita Porter 🙋🏻‍♀️The distribution of genetic diversity in ecological communities: A unifying measure for monitoring biodiversity change<a href="https://ecoevorxiv.org/repository/view/9169/" rel="nofollow noopener" translate="no" target="_blank">https://ecoevorxiv.org/repository/view/9169/</a>TLDR - CGD - distribution of nucleotide diversity values across all OTUs in a community sample<a href="https://ecoevo.social/tags/CommunityGeneticDistribution" class="mention hashtag" rel="nofollow noopener" target="_blank">#CommunityGeneticDistribution</a> <a href="https://ecoevo.social/tags/CGD" class="mention hashtag" rel="nofollow noopener" target="_blank">#CGD</a> <a href="https://ecoevo.social/tags/biodiversity" class="mention hashtag" rel="nofollow noopener" target="_blank">#biodiversity</a> <a href="https://ecoevo.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> <a href="https://ecoevo.social/tags/OTU" class="mention hashtag" rel="nofollow noopener" target="_blank">#OTU</a>

Teresita Porter 🙋🏻‍♀️Capture—Incubate—Release: An Animal-Friendly Approach to Assess Local Aquatic Macroinvertebrate Species Diversity Through Environmental DNA Metabarcoding<a href="https://onlinelibrary.wiley.com/doi/10.1002/edn3.70112" rel="nofollow noopener" translate="no" target="_blank">https://onlinelibrary.wiley.com/doi/10.1002/edn3.70112</a><a href="https://ecoevo.social/tags/eDNA" class="mention hashtag" rel="nofollow noopener" target="_blank">#eDNA</a> <a href="https://ecoevo.social/tags/macroinvertebrate" class="mention hashtag" rel="nofollow noopener" target="_blank">#macroinvertebrate</a> <a href="https://ecoevo.social/tags/nondestructive" class="mention hashtag" rel="nofollow noopener" target="_blank">#nondestructive</a> <a href="https://ecoevo.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a>

Lukas VFN 🇪🇺Researchers partner with <a href="https://scholar.social/tags/IndigenousKnowledge" class="mention hashtag" rel="nofollow noopener" target="_blank">#IndigenousKnowledge</a> holders to protect threatened great desert skink Tjakuṟa <a href="https://phys.org/news/2025-01-partner-indigenous-knowledge-holders-threatened.html" rel="nofollow noopener" translate="no" target="_blank">https://phys.org/news/2025-01-partner-indigenous-knowledge-holders-threatened.html</a><a href="https://scholar.social/tags/Metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#Metabarcoding</a> clarifies the diet of the elusive and vulnerable Australian <a href="https://scholar.social/tags/tjakura" class="mention hashtag" rel="nofollow noopener" target="_blank">#tjakura</a> (Great Desert <a href="https://scholar.social/tags/Skink" class="mention hashtag" rel="nofollow noopener" target="_blank">#Skink</a>, Liopholis kintorei) <a href="https://www.frontiersin.org/journals/ecology-and-evolution/articles/10.3389/fevo.2024.1354138/full" rel="nofollow noopener" translate="no" target="_blank">https://www.frontiersin.org/journals/ecology-and-evolution/articles/10.3389/fevo.2024.1354138/full</a>"Tjakuṟa is a large <a href="https://scholar.social/tags/lizard" class="mention hashtag" rel="nofollow noopener" target="_blank">#lizard</a> that lives in multi-tunneled communal burrows in <a href="https://scholar.social/tags/Australia" class="mention hashtag" rel="nofollow noopener" target="_blank">#Australia</a>... scientists rely on <a href="https://scholar.social/tags/A%E1%B9%89angu" class="mention hashtag" rel="nofollow noopener" target="_blank">#Aṉangu</a> Traditional Knowledge to locate Tjakuṟa burrows, estimate burrow occupancy, identify predator tracks"

Teresita Porter 🙋🏻‍♀️Developing and testing a new Ecological Quality Status index based on marine nematode metabarcoding: A proof of concept<a href="https://www.sciencedirect.com/science/article/pii/S004565352402900X" rel="nofollow noopener" translate="no" target="_blank">https://www.sciencedirect.com/science/article/pii/S004565352402900X</a><a href="https://ecoevo.social/tags/biomonitoring" class="mention hashtag" rel="nofollow noopener" target="_blank">#biomonitoring</a> <a href="https://ecoevo.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> <a href="https://ecoevo.social/tags/nematodes" class="mention hashtag" rel="nofollow noopener" target="_blank">#nematodes</a> <a href="https://ecoevo.social/tags/marine" class="mention hashtag" rel="nofollow noopener" target="_blank">#marine</a>

Teresita Porter 🙋🏻‍♀️ITSxpress version 2: software to rapidly trim internal transcribed spacer sequences with quality scores for amplicon sequencing<a href="https://journals.asm.org/doi/10.1128/spectrum.00601-24" rel="nofollow noopener" translate="no" target="_blank">https://journals.asm.org/doi/10.1128/spectrum.00601-24</a><a href="https://ecoevo.social/tags/ITS" class="mention hashtag" rel="nofollow noopener" target="_blank">#ITS</a> <a href="https://ecoevo.social/tags/rDNA" class="mention hashtag" rel="nofollow noopener" target="_blank">#rDNA</a> <a href="https://ecoevo.social/tags/trimming" class="mention hashtag" rel="nofollow noopener" target="_blank">#trimming</a> <a href="https://ecoevo.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> <a href="https://ecoevo.social/tags/bioinformatics" class="mention hashtag" rel="nofollow noopener" target="_blank">#bioinformatics</a>

Teresita Porter 🙋🏻‍♀️A new spike-in-based method for quantitative metabarcoding of soil fungi and bacteria<a href="https://link.springer.com/article/10.1007/s10123-023-00422-5" rel="nofollow noopener" translate="no" target="_blank">https://link.springer.com/article/10.1007/s10123-023-00422-5</a><a href="https://ecoevo.social/tags/quantitative" class="mention hashtag" rel="nofollow noopener" target="_blank">#quantitative</a> <a href="https://ecoevo.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> <a href="https://ecoevo.social/tags/bacteria" class="mention hashtag" rel="nofollow noopener" target="_blank">#bacteria</a> <a href="https://ecoevo.social/tags/fungi" class="mention hashtag" rel="nofollow noopener" target="_blank">#fungi</a>

Teresita Porter 🙋🏻‍♀️What Quality Suffices for Nanopore Metabarcoding? Reconsidering Methodology and Ectomycorrhizae in Decaying *Fagus sylvatica* Bark as Case Study<a href="https://www.mdpi.com/2309-608X/10/10/708" rel="nofollow noopener" translate="no" target="_blank">https://www.mdpi.com/2309-608X/10/10/708</a><a href="https://ecoevo.social/tags/nanopore" class="mention hashtag" rel="nofollow noopener" target="_blank">#nanopore</a> <a href="https://ecoevo.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> <a href="https://ecoevo.social/tags/fungi" class="mention hashtag" rel="nofollow noopener" target="_blank">#fungi</a> <a href="https://ecoevo.social/tags/ECM" class="mention hashtag" rel="nofollow noopener" target="_blank">#ECM</a> <a href="https://ecoevo.social/tags/ectomycorrhae" class="mention hashtag" rel="nofollow noopener" target="_blank">#ectomycorrhae</a>

Lukas VFN 🇪🇺Saving the <a href="https://scholar.social/tags/bats" class="mention hashtag" rel="nofollow noopener" target="_blank">#bats</a>: Researchers find <a href="https://scholar.social/tags/bacteria" class="mention hashtag" rel="nofollow noopener" target="_blank">#bacteria</a> and <a href="https://scholar.social/tags/fungi" class="mention hashtag" rel="nofollow noopener" target="_blank">#fungi</a> on <a href="https://scholar.social/tags/bat" class="mention hashtag" rel="nofollow noopener" target="_blank">#bat</a> wings that could help fight deadly white-nose syndrome <a href="https://phys.org/news/2024-10-bacteria-fungi-wings-deadly-white.html" rel="nofollow noopener" translate="no" target="_blank">https://phys.org/news/2024-10-bacteria-fungi-wings-deadly-white.html</a>DNA <a href="https://scholar.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> analyses reveal fine-scale <a href="https://scholar.social/tags/microbiome" class="mention hashtag" rel="nofollow noopener" target="_blank">#microbiome</a> structures on Western Canadian bat wings <a href="https://journals.asm.org/doi/10.1128/spectrum.00376-24" rel="nofollow noopener" translate="no" target="_blank">https://journals.asm.org/doi/10.1128/spectrum.00376-24</a> "Healthy wings are critical for the survival and reproduction of bats and the wing microbiome is believed to play a major role in their susceptibility to <a href="https://scholar.social/tags/WhiteNoseSyndrome" class="mention hashtag" rel="nofollow noopener" target="_blank">#WhiteNoseSyndrome</a>"

Teresita Porter 🙋🏻‍♀️Environmental RNA metabarcoding for ballast water microbial diversity: Minimizing false positives<a href="https://doi.org/10.1016/j.scitotenv.2024.176902" rel="nofollow noopener" translate="no" target="_blank">https://doi.org/10.1016/j.scitotenv.2024.176902</a><a href="https://ecoevo.social/tags/eDNA" class="mention hashtag" rel="nofollow noopener" target="_blank">#eDNA</a> <a href="https://ecoevo.social/tags/eRNA" class="mention hashtag" rel="nofollow noopener" target="_blank">#eRNA</a> <a href="https://ecoevo.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> <a href="https://ecoevo.social/tags/biomonitoring" class="mention hashtag" rel="nofollow noopener" target="_blank">#biomonitoring</a>

Teresita Porter 🙋🏻‍♀️The ASV Registry: a place for ASVs to be<a href="https://mbmg.pensoft.net/article/124564/" rel="nofollow noopener" translate="no" target="_blank">https://mbmg.pensoft.net/article/124564/</a><a href="https://ecoevo.social/tags/DNA" class="mention hashtag" rel="nofollow noopener" target="_blank">#DNA</a> <a href="https://ecoevo.social/tags/Metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#Metabarcoding</a> <a href="https://ecoevo.social/tags/ESV" class="mention hashtag" rel="nofollow noopener" target="_blank">#ESV</a> <a href="https://ecoevo.social/tags/ASV" class="mention hashtag" rel="nofollow noopener" target="_blank">#ASV</a> <a href="https://ecoevo.social/tags/ZOTU" class="mention hashtag" rel="nofollow noopener" target="_blank">#ZOTU</a> <a href="https://ecoevo.social/tags/database" class="mention hashtag" rel="nofollow noopener" target="_blank">#database</a>

Lukas VFN 🇪🇺New study reveals the crucial role of <a href="https://scholar.social/tags/jellyfish" class="mention hashtag" rel="nofollow noopener" target="_blank">#jellyfish</a> in Greenlandic waters <a href="https://www.rcinet.ca/eye-on-the-arctic/2024/08/27/new-study-reveals-the-crucial-role-of-jellyfish-in-greenlandic-waters/" rel="nofollow noopener" translate="no" target="_blank">https://www.rcinet.ca/eye-on-the-arctic/2024/08/27/new-study-reveals-the-crucial-role-of-jellyfish-in-greenlandic-waters/</a>A belly full of jelly? DNA <a href="https://scholar.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> shows evidence for gelatinous <a href="https://scholar.social/tags/zooplankton" class="mention hashtag" rel="nofollow noopener" target="_blank">#zooplankton</a> predation by several <a href="https://scholar.social/tags/fish" class="mention hashtag" rel="nofollow noopener" target="_blank">#fish</a> species in <a href="https://scholar.social/tags/Greenland" class="mention hashtag" rel="nofollow noopener" target="_blank">#Greenland</a> waters <a href="https://royalsocietypublishing.org/doi/10.1098/rsos.240797" rel="nofollow noopener" translate="no" target="_blank">https://royalsocietypublishing.org/doi/10.1098/rsos.240797</a>"Scientists have commonly regarded jellyfish as a secondary food source for predatory fish, due to their low energy density and nutritional value. But... fish consume jellyfish much more frequently than previously thought."

Teresita Porter 🙋🏻‍♀️The development of terrestrial ecosystems emerging after glacier retreat<a href="https://www.nature.com/articles/s41586-024-07778-2" rel="nofollow noopener" translate="no" target="_blank">https://www.nature.com/articles/s41586-024-07778-2</a><a href="https://ecoevo.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> <a href="https://ecoevo.social/tags/diversity" class="mention hashtag" rel="nofollow noopener" target="_blank">#diversity</a> <a href="https://ecoevo.social/tags/ecosystem" class="mention hashtag" rel="nofollow noopener" target="_blank">#ecosystem</a>

Teresita Porter 🙋🏻‍♀️Airborne DNA reveals predictable spatial and seasonal dynamics of fungi<a href="https://www.nature.com/articles/s41586-024-07658-9" rel="nofollow noopener" translate="no" target="_blank">https://www.nature.com/articles/s41586-024-07658-9</a><a href="https://ecoevo.social/tags/fungi" class="mention hashtag" rel="nofollow noopener" target="_blank">#fungi</a> <a href="https://ecoevo.social/tags/mycolology" class="mention hashtag" rel="nofollow noopener" target="_blank">#mycolology</a> <a href="https://ecoevo.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a>

Teresita Porter 🙋🏻‍♀️Nonparametric richness estimators Chao1 and ACE must not be used with amplicon sequence variant data<a href="https://doi.org/10.1093/ismejo/wrae106" rel="nofollow noopener" translate="no" target="_blank">https://doi.org/10.1093/ismejo/wrae106</a><a href="https://ecoevo.social/tags/BiodiversityEstimators" class="mention hashtag" rel="nofollow noopener" target="_blank">#BiodiversityEstimators</a> <a href="https://ecoevo.social/tags/Chao1" class="mention hashtag" rel="nofollow noopener" target="_blank">#Chao1</a> <a href="https://ecoevo.social/tags/ACE" class="mention hashtag" rel="nofollow noopener" target="_blank">#ACE</a> <a href="https://ecoevo.social/tags/ASVs" class="mention hashtag" rel="nofollow noopener" target="_blank">#ASVs</a> <a href="https://ecoevo.social/tags/AmpliconSequenceVariants" class="mention hashtag" rel="nofollow noopener" target="_blank">#AmpliconSequenceVariants</a> <a href="https://ecoevo.social/tags/Metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#Metabarcoding</a>

Teresita Porter 🙋🏻‍♀️Taxonomic accuracy and complementarity between bulk and eDNA metabarcoding provides an alternative to morphology for biological assessment of freshwater macroinvertebrates<a href="https://doi.org/10.1016/j.scitotenv.2024.173243" rel="nofollow noopener" translate="no" target="_blank">https://doi.org/10.1016/j.scitotenv.2024.173243</a><a href="https://ecoevo.social/tags/bulk" class="mention hashtag" rel="nofollow noopener" target="_blank">#bulk</a> <a href="https://ecoevo.social/tags/eDNA" class="mention hashtag" rel="nofollow noopener" target="_blank">#eDNA</a> <a href="https://ecoevo.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> <a href="https://ecoevo.social/tags/freshwater" class="mention hashtag" rel="nofollow noopener" target="_blank">#freshwater</a> <a href="https://ecoevo.social/tags/macroinvertebrates" class="mention hashtag" rel="nofollow noopener" target="_blank">#macroinvertebrates</a>

Teresita Porter 🙋🏻‍♀️Measuring the state of aquatic environments using eDNA—upscaling spatial resolution of biotic indices<a href="https://royalsocietypublishing.org/doi/10.1098/rstb.2023.0121" rel="nofollow noopener" translate="no" target="_blank">https://royalsocietypublishing.org/doi/10.1098/rstb.2023.0121</a><a href="https://ecoevo.social/tags/eDNA" class="mention hashtag" rel="nofollow noopener" target="_blank">#eDNA</a> <a href="https://ecoevo.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> <a href="https://ecoevo.social/tags/BioticIndices" class="mention hashtag" rel="nofollow noopener" target="_blank">#BioticIndices</a>

Lukas VFN 🇪🇺Research team shows island <a href="https://scholar.social/tags/bats" class="mention hashtag" rel="nofollow noopener" target="_blank">#bats</a> are valuable allies for farmers <a href="https://phys.org/news/2024-04-team-island-valuable-allies-farmers.html" rel="nofollow noopener" translate="no" target="_blank">https://phys.org/news/2024-04-team-island-valuable-allies-farmers.html</a>A <a href="https://scholar.social/tags/metabarcoding" class="mention hashtag" rel="nofollow noopener" target="_blank">#metabarcoding</a> assessment of the diet of the insectivorous bats of <a href="https://scholar.social/tags/Madeira" class="mention hashtag" rel="nofollow noopener" target="_blank">#Madeira</a> Island, <a href="https://scholar.social/tags/Macaronesia" class="mention hashtag" rel="nofollow noopener" target="_blank">#Macaronesia</a>: Angelina Gonçalves et al. <a href="https://academic.oup.com/jmammal/advance-article/doi/10.1093/jmammal/gyae033/7642761" rel="nofollow noopener" translate="no" target="_blank">https://academic.oup.com/jmammal/advance-article/doi/10.1093/jmammal/gyae033/7642761</a>"all three <a href="https://scholar.social/tags/bat" class="mention hashtag" rel="nofollow noopener" target="_blank">#bat</a> species had highly diverse diets—eating over 50 different species among them, including <a href="https://scholar.social/tags/beetles" class="mention hashtag" rel="nofollow noopener" target="_blank">#beetles</a>, <a href="https://scholar.social/tags/butterflies" class="mention hashtag" rel="nofollow noopener" target="_blank">#butterflies</a>, <a href="https://scholar.social/tags/flies" class="mention hashtag" rel="nofollow noopener" target="_blank">#flies</a>, <a href="https://scholar.social/tags/moths" class="mention hashtag" rel="nofollow noopener" target="_blank">#moths</a>, and <a href="https://scholar.social/tags/spiders" class="mention hashtag" rel="nofollow noopener" target="_blank">#spiders</a>. Overall, 40% (23) of the identified species were likely or confirmed agricultural or forestry <a href="https://scholar.social/tags/pests" class="mention hashtag" rel="nofollow noopener" target="_blank">#pests</a>"

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